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Seinfeld episodes were made available for streaming on Amazon Prime for the first time in 2017. The sitcom from the 1990s remains popular and relevant today, and yet no one has analysed how it portrays science. This article begins that analysis by investigating how Seinfeld represents science. A surprisingly large number of nuanced references to science and scientific expertise appear across the series. Seinfeld did not shy away from including science and even scientific jargon into its narratives, some of which revolve around a scientific topic. Seinfeld presents a rich source for studying science in popular culture, one that has so far been neglected but that merits study due to its continuing fame and the popular influence of the series.